MG1655
W3110
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Gene Report : lpxB
PEC Original Annotations
Essentiality
     Class essential
     References (PMID)
J Bacteriol. 1987;169(12):5735-44.
Sequence analysis of the Escherichia coli dnaE gene.
Tomasiewicz HG, McHenry CS. ( 3316192 )
     Deletion OCR33,35-4 (AK4-2 / BK5-2) (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  lpxB
Related strains
     Strains Search Search strains by gene name:  lpxB   Search strains by all related name:  lpxB b0182 ECK0181 JW0177 pgsB

General information  (Go to Linear View:)
 Gene Name lpxB  
 Alternative name b0182,ECK0181,JW0177,pgsB  
 Location, Length 203,348 - 204,496 (  +  ) ;   4.38 min ; 1149 (bp) ,   382 (aa) Go to Linear View
 Product tetraacyldisaccharide-1-P synthase  
 Operon Name bamA-hlpA-lpxD-fabZ-lpxAB-rnhB-dnaE  
 Note tetraacyldisaccharide-1-P; lipid A biosynthesis, penultimate step; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0005737 - cytoplasm; GO_function: GO:0042280 - cell surface antigen activity, host-interacting; GO_process: GO:0009245 - lipid A biosynthetic process  
 Function enzyme; Macromolecule metabolism: Lipopolysaccharide  
 Gene Ontology GO:0005515 ; protein binding ( lpxB )
GO:0008610 ; lipid biosynthetic process ( lpxB )
GO:0008915 ; lipid-A-disaccharide synthase activity ( lpxB )
GO:0009245 ; lipid A biosynthetic process ( lpxB )
GO:0016740 ; transferase activity ( lpxB )
GO:0016757 ; transferase activity, transferring glycosyl groups ( lpxB )
 PID 1786379  
 EC number
  (KEGG Pathway)   		2.4.1.182  
SWISS-PROT  ( Show details [ 2 more] )
  botton Entry name(Acc.no) LPXB_ECOLI ( P10441 ; P78298 )
    -  Protein name Lipid-A-disaccharide synthase  
    -  Synonyms EC 2.4.1.182  
    -  Gene name Name=lpxB; Synonyms=pgsB; OrderedLocusNames=b0182;  

 Linear View (Whole Mode)
View Location
   195.0  –  220.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP lpxB (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Structure At 2.7 Angstrom Resolution Of The Paracoccus Denitrificans Two-Subunit Cytochrome C Oxidase Complexed With An Antibody Fv Fragment  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Lipid-A-disaccharide synthase  
         PSI-BLAST Lipid-A-disaccharide synthase
    nr     (database updated : 2007.02.20 )
         BLAST lipid-A-disaccharide synthase [Escherichia coli K12]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL     

Other Cross-Reference
    COG COG0763M 
    EcoCyc lpxB 

MMBR References
J Bacteriol. 1987;169(12):5735-44.
Sequence analysis of the Escherichia coli dnaE gene.
Tomasiewicz HG, McHenry CS. ( 3316192 )
J Bacteriol. 1988;170(3):1268-74.
First committed step of lipid A biosynthesis in Escherichia coli: sequence of the lpxA gene.
Coleman J, Raetz CRH. ( 3277952 )
J Bacteriol. 1986;168(1):152-9.
Molecular cloning of the genes for lipid A disaccharide synthase and UDP-N-acetylglucosamine acyltransferase in Escherichia coli.
Crowell DN, Anderson MS, Raetz CRH. ( 3531165 )
J Bacteriol. 1987;169(12):5727-34.
Nucleotide sequence of the Escherichia coli gene for lipid A disaccharide synthase.
Crowell DN, Reznikoff WS, Raetz CRH. ( 2824445 )

Sequences
Amino acid
FASTA format 
0001 MTEQRPLTIA LVAGETSGDI LGAGLIRALK EHVPNARFVG VAGPRMQAEG CEAWYEMEEL AVMGIVEVLG 
0071 RLRRLLHIRA DLTKRFGELK PDVFVGIDAP DFNITLEGNL KKQGIKTIHY VSPSVWAWRQ KRVFKIGRAT 
0141 DLVLAFLPFE KAFYDKYNVP CRFIGHTMAD AMPLDPDKNA ARDVLGIPHD AHCLALLPGS RGAEVEMLSA 
0211 DFLKTAQLLR QTYPDLEIVV PLVNAKRREQ FERIKAEVAP DLSVHLLDGM GREAMVASDA ALLASGTAAL 
0281 ECMLAKCPMV VGYRMKPFTF WLAKRLVKTD YVSLPNLLAG RELVKELLQE ECEPQKLAAA LLPLLANGKT 
0351 SHAMHDTFRE LHQQIRCNAD EQAAQAVLEL AQ
Nucleotide
FASTA format

View sequence out neighbor 100bp 
-100                                             ATGAAGTGAA ACCGGAAATT GCTGAACTGG 
-070 CGGAAACATA TCCGGAAGTG AAAGCCTTTA CCGATTTCTT TGCACGCTCA ACGCGCGGTC TGATTCGTTA 
0001 atgactgaac agcgtccatt aacgattgcc ctggtcgccg gagaaacctc cggcgatatc ctgggggccg 
0071 gtttaatccg cgctctgaaa gaacatgtgc ccaacgcccg ctttgttggt gttgccgggc cacgaatgca 
0141 ggctgaaggc tgcgaagcct ggtacgaaat ggaagaactg gcggtgatgg gcattgttga agtgctcggt 
0211 cgtctgcgtc gcttactgca tattcgtgcc gatctgacaa agcgttttgg cgaactgaag ccagatgttt 
0281 ttgttggtat tgatgcgcct gacttcaata ttactcttga aggtaacctc aaaaagcagg gtatcaaaac 
0351 cattcattac gtcagtccgt cagtctgggc gtggcgacag aaacgtgttt tcaaaatagg cagagccacc 
0421 gatctggtgc tcgcatttct gcctttcgaa aaagcgtttt atgacaaata caacgtaccg tgccgcttta 
0491 tcggtcatac catggctgat gccatgccat tagatccaga taaaaatgcc gcccgtgatg tgctggggat 
0561 ccctcacgat gcccactgcc tggcgttgct accggggagc cgtggtgcag aagttgaaat gcttagtgcc 
0631 gatttcctga aaacggccca gcttttgcgc cagacatatc cggatctcga aatcgtggtg ccactggtga 
0701 atgccaaacg ccgcgagcag tttgaacgca tcaaagctga agtcgcgcca gacctttcag ttcatttgct 
0771 ggatgggatg ggccgtgagg cgatggtcgc cagcgatgcg gcgctactgg cgtcgggtac ggcagccctg 
0841 gagtgtatgc tggcgaaatg cccgatggtg gtgggatatc gcatgaagcc ttttaccttc tggttggcga 
0911 agcggctggt gaaaactgat tatgtctcgc tgccaaatct gctggcgggc agagagttag tcaaagaatt 
0981 attgcaggaa gagtgtgagc cgcaaaaact ggctgcggcg ctgttaccgc tgttggcgaa cgggaaaacc 
1051 agccacgcga tgcacgatac cttccgtgaa ctgcatcagc agatccgctg caatgccgat gagcaggcgg 
1121 cacaagccgt tctggagtta gcacaatgaT CGAATTTGTT TATCCGCACA CGCAGCTGGT TGCGGGTGTG 
1191 GATGAAGTCG GACGCGGGCC GTTAGTTGGC GCGGTCGTCA CCGCTGCGGT GATCCTTGA