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A. Saito1 and
Y. Ogihara2 (1Kyusyu National Agric. Exp.
Station, 2Kihara Inst. Biol. Res., Yokohama, City
Univ.)
Specificity of genomes in cereal plants: some motifs of repetitive
sequence in the Gramineae.
A number of cereal molecular maps were constructed as based on
RFLP markers. We characterized some rice RFLP markers to be
homologous or heterologous to other cereal genomes. We found that
some regions in rice genome might be putative ancestral genome in
cereal. On the other hand, four RFLP markers being a few copied
clones exhibited moderately repetitive sequences in most of the
Gramineae. Northern analyses revealed these clones to be expressed in
cereal leaves. These sequences had no homology to each
other and reported genes. RNA folding analyses, revealed that all
four clones contain remarkable stem (loop) structure in their
sequences.
K. Nishikawa (Kihara Mem. Yokohama Found. Adv. Life
Sci.)
Planning on a new network for wheat genetic resources
Agreements at the meeting held three times in 1994 for planning a
new network for wheat genetic resources were reported as follows: 1)
The network is organized from a center and stations, 2) it is
concerned only genetic and experimental lines of Triticum,
Aegilops and
Avena, 3)
The center operates general amnagement, 4) Station does registration,
actual preservation, distribution, and assembling and updating data
base, of the lines, 5) DNA clones are expected to be included in the
network. Nineteen institutes or laboratories have agreed
participation as a station in the network.
Y. Yamazaki (Genetic Stocks Res. Center, National Inst. of
Genetics)
Wheat genetic resources database in Japan
The first phase of the Genetic Resources Databank Project for
wheat has been initiated in this laboratory in cooperation with Wheat
Networking Group of Japan. The database is composed of ca. 70
entities including the biological and molecular biological features
of each strain, as well as bibliographical information. The working
group plans to continue the work on the database in several
directions, such as incorporation of the wheat DNA repository
database and of image data and cross referencing with related
databases. The development of a data management system through which
researcher can constantly update their own data by connecting to the
remote computer runnning the databases is also an ongoing
project.
Y. Ogihara (Kihara Inst. Biol. Res., Yokohama City Univ.)
DNA repository in wheat
It has been well recognized that DNA repository is essential not
only for practical use, but also for basic research work. In order to
set up DNA repository for wheat species, I presented and discussed
on, what kinds of DNAs should be collected, and how those DNAs could
be maintained, in connection to genome database of Japan. Based on
the assessment of DNA clones related to wheat species 'm Japan, I
have written to the researchers who keep the wheat DNA clones and/or
DNA itself, so as to register those information. At the next step,
DNA repository office should collect those DNA materials and maintain
in the DNA repository center.
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