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Wheat Information Service
Number 86: 25-30 (1998)
Research article

Chromosome assignment and polymorphism of a wheat cDNA encoding protein disulfide isomerase

G. Segal1, Y. Shimoni1, H. Li1 and S. Abbo2*

1Department of Plant Sciences, The Weizmann Institute of Science, Rehovot 76100, Israel

2Department of Field Crops, Vegetables and Genetics, Faculty of Agriculture, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, Rehovot 76100, Israel


Summary

Chromosomal location, cytological mapping and the assessment of variability of a cDNA encoding protein disulfide isomerase (PDI) in wheat are reported. Three PDI homoeoloci were mapped to chromosome arms 4AL, 4BS and 4DS. Deletion mapping localized PDI sequences at the distal 0.3 length fraction of 4DS, in a region, thus far, poor in other DNA markers. RFLP pattern detected by PDI probes were extremely conserved among hexaploid wheats. Some polymorphism was observed among tetraploid wild wheats, and among A-genome and S-genome diploids, making PDI probes useful to monitor introgressions from these lines into common wheat.

Key words: PDI (protein disulfide isomerase), cytological mapping, Triticum sp., Aegilops sp.


Introduction

Mapping of plant genomes has been attracting considerable attention in the past two decades (reviewed by Dean and Schmidt 1995). In this respect, combining the recombinational and cytological maps is of particular importance (Warner et al. 1992). Inspection of a number of cytological maps of wheat chromosomes reveals that considerable length fractions (up to 15%) currently lack cloned DNA markers, while other chromosomal regions are relatively rich in such markers (Hohmann et al. 1994; Mickelson-Young et al. 1995; Delaney et al. 1995). Therefore, it is important to obtain new chromosomal landmarks for regions in which DNA markers are scarce.

Protein disulfide isomerase (PDI EC 5.3.4. 1) is an enzyme involved in the formation of disulfide bonds required for correct protein folding (Shimoni et al. 1995). Following its recent cloning and characterization in commonwheat (therein) we hereby describe its cytological location and possible usefulness as a genetic marker in wheat.
*Corresponding author: Tel: 972-8-9481943 Fax: 972-8-9468265 e-mail: abbo@agri.huji.ac.il

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