In the study, 21 accessions of 7 different Aegilops species, from 3 different altitudes were collected throughout Turkey. The species, regions and altitudes from which the samples were collected, were given in Table 1 and Fig. 1.
The seeds were germinated at the greenhouse and transplanted into the field when they were at tillering stage to provide seeds needed for analysis of protein patterns. Electrophoresis methods suggested by Bushuk and Zillman (1978) and Tkachuk and Metlish (1980) were applied. Some modifications were done (Khan et al. 1985; Lookhart et al. 1982) for increasing the strength and decreasing the stickiness of the gel. After dying the gel, 70% alcohol was added in varying amounts depending on the species to get a clear vision of the bands. The amount of gliadin sample solution taken, varied among 8-10 micro-liter.
Seeds were ground, 70% alcohol was added and centrifuged. Extraction solution was added to the supernatant- gliadin solution (Burgoon et al. 1985; Metakovsky and Baboev 1992). Electrophoresis buffer solution (Maier and Wagner 1980; Metakovsky and Novaselskaya, 1991), extract dilution solution, gel solution (Khan 1982), dying solution (Ewart 1973; Zillman and Bushuk 1979) and dye-removal solution were prepared and applied on a one dimensional electrophoresis apparatus with double gel cassettes, and a dual vertical slab gel mechanism.
Results were evaluated on 9 x 13 cm size photographs (Kosmolak et al. 1980). Relative mobility (Rm) values were calculated using Marquis species as a standard (Bushuk and Zillman 1978). A well-separated band of the electrophoregram. was marked as the Rm reference values of 50 and the Rm values of the other bands were calculated according to the reference. To calculate the Rm value of a specific band, the distance of this band from the origin was divided into the distance of the Marquis reference band and then the value obtained was multiplied by the factor of 50. Intensity readings of the bands were taken and assessed on a 1-5 scale, 1 being the lightest and 5 the darkest (Draper and Craig 1981). Relative mobility and relative intensity (Ri) values of each sample were listed and formulas of species were obtained. By using Rm values on species list (French system used by Bushuk and Zillman 1978) Rm values were classified between 0-59 for omega gliadin region, between 59-74 for gamma gliadin region, 74-85 for beta gliadin region and 85-100 for alpha gliadin regions (Motel and Mayer 1981; Lookhart et al. 1983).